Safiyeh Ghazzafi; Ahmad Hamta
Volume 21, Issue 1 , March and April 2015, , Pages 122-130
Abstract
Background: Breast Cancer is the most common cancer in the Iranian women. Fortunately, it is a type of preventable and discernible cancer. The hydroethanolic extract of the leaves and flowered twigs of Thymu vulgaris contain various ingrediants in which the Thymol and Carvacrol are the most important ...
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Background: Breast Cancer is the most common cancer in the Iranian women. Fortunately, it is a type of preventable and discernible cancer. The hydroethanolic extract of the leaves and flowered twigs of Thymu vulgaris contain various ingrediants in which the Thymol and Carvacrol are the most important of them. The effects of this anti-cancer in this extract are attributed significantly to these ingrediants . The objective of this research is to study the comparison of the efficiency of hydroethanolic extract and taxol on 4T1 line cells and their effects rate on the induction of apoptosis in cancer cells.
Materials and Methods: In this research, 4T1 line cells were bought from Tehran's Pasteur institute and the hydroethanolic extract is provided by the Soxhlet’s method. Therefore, We obtained Cytotoxicity five different doses of the extract and five different doses of Taxol in 24, 48 and 72 hours and studied the viability of cells by the MTT and Tripan blue methods. Also, in this research the morphologic changes are done by the Hoechst and Propidiumi odied coloring methods.
Results: Extract and taxol-treated cells at different times have indicated distinct differences and dose-related results. Therefore, simultaneous treatment with the extract in comparison with taxol and taxol-treated cell extract can destroy more cells.
Conclusion: The hydroethanolic extract can increase the cytotoxicity Taxol, Thus it maybe be effective in breast cancer remedy and probably by apoptosis Induction may cause cells death.